BIOCON®
INDEPENDENT LAB TEST RESULTS


The laboratories conducting these tests were commissioned by various companies involved in the original formulation and manufacture of the Copper-Zinc filter media known as KDF. Each test describes the purpose and procedure used as well as the results. Please contact us for complete documentation on any of the tests shown here.

Biological Research Solutions, Inc.
Detroit, Michigan

Laboratory Repor
t

Client: KDF Fluid Treatment, Inc.
Samples Received: 01-21-91
Lab Reference No: IV-79

Laboratory Service Performed: Evaluation of the Bacteriostatic Properties of a Water Treatment Device Containing an Alloy of Copper and Zinc (KDF).

I. Introduction:

Water treatment devices may be colonized by bacteria and thus promote the growth of bacteria contained in the influent water. It is important, therefore, that devices that are intended for use in potable water supplies be tested to determine if they are bacteriostatic (i.e. do not stimulate the growth of microorganisms).

II. Procedures:

The testing protocol used is described in Table 1. This procedure provides a heavy bacterial challenge over a seven day period followed by a period of time to allow the growth of the challenge bacteria. If the treatment device provides an environment that is favorable for bacterial growth, there should be a demonstrable increase in bacterial numbers during the latter stages of the test.

In order to test the device, [a] model water system … was used. The supply water for the model system was chlorine free deionized water that was reconstructed to approximate Detroit city water by the injection of AOAC synthetic hard water to achieve a final concentration of 100 ppm and a pH value of 7.0.

A fresh suspension of Enterobacter aerogenes (former name Aerobacter aerogenes) was prepared daily and injected into the flowing water stream to achieve a final bacterial concentration of 300 cfu/ml.

III. Results and Discussion:

The results of the 19 day bacterial challenge test are shown in Table 2. For the first seven days Enterobacter was injected into the influent water stream and the survivors that passed through the test device were measured. The were endogenous bacteria (naturally occurring water bacteria other than Enterobacter) present in the model water system at the beginning of the testing, and there was a large reduction in these bacteria as a result of passage through the water treatment device. The results shown in Table 2 for the first seven days of testing show the Enterobacter survivors only. The measurements after 11 and 19 days, during which time no additional Enterobacter was injected into the system, the results in Table 2 show the percent reduction in these endogenous bacteria (no Enterobacter survivors were detected).

This testing showed that the water treatment device is bacteriostatic and does not stimulate the growth of Enterobacter nor does it provide an environment that promotes survival. In addition, the device is effective in reducing the concentration of Enterbacter or other endogenous bacteria present in the influent water (bacteriocidal effect) and thus may be useful in the control of potentially harmful bacteria that may inadvertently enter potable water supplies.

Biological Research Solutions, Inc.

By: John W. Wireman, Ph.D.

Dated: February 25, 1991

Table 1
Description of Test Procedure
Standard Conditions:

1 - Test Microorganism: Enterobacter aerogenes
2 - Concentration of Test Microorganisms: 300 cfu/ml
3 - Flow Rate: 1.0 – 1.5 liters per minute
4 - Water Hardness: 100 ppm

Test Day
Backwash
Purge
Bacteria Injected
Survivor Measurement
1
None
None
Yes
Every 10 liters for 100 liter total flow
2-6
None
None
Yes
Every 10 liters for 20 liter total flow
7
15 Min
10 Min
Yes
After 10 liter flow
11
None
None
No
Every 10 liters for 20 liter total flow
19
15 Min
10 Min
No
Every 10 liters for 20 liter total flow

Table 2
Results of Water Treatment Device Evaluation

Total Water flow (1)
10
20
30
40
50
60
70
80
90
100
Enterobacter aerogenes Survivors (cfu/ml)
DAY 1
6
4
5
5
2
3
5
5
8
8
DAY 2
6
10
DAY 3
4
4
DAY 4
24
7
DAY 5
1
0
DAY 6
0
2
DAY 7
2
TOTAL WATER FLOW (1)
10
20
Reduction in Endogenous Bacteria (5)
DAY 11
80%
73%
DAY 19
59%
30%

No Enterobacter aerogenes survivors were detectable on Day 11 or 19

Biological Research Solutions, Inc.

By: John W. Wireman, Ph.D.

Dated: February 25, 1991


Terralab Engineers
Salt Lake City, UT

Analysis No: 15712
Report Date: 4 Dec 1898

This is to certify that we have examined:
Water Filtration Unit identified as WaterQuest Countertop0 (CT) to the requirements of NSF 53

and found:
When tested to the requirements of the applicable paragraphs of the National Sanitation Foundation Standard No. 53, "Drinking Water Treatment Units - Health Effects" the ... unit met the applicable requirements of the standard.

The attached Report of Test is an integral part of this Certificate.

Terralab by
Douglas MacGregor

NSF Standard 53
Drinking Water
Treatment Units
Health Effects

REPORT OF TEST
15712
of
WATEROUEST, INC.
WATERQUEST COUNTERTOP (CT) WATER FILTRATION UNIT
CLIENT: WATERQUEST. INC.

Water filtration unit tested to the requirements of National Sanitation Foundation (NSF) Standard Number 53, Drinking Water Treatment Units, Health Effects

CAVEAT
NOTE: The values given in this report are the values obtained under standard test conditions and thus may be used for purposes of determining compliance or for comparison with other units tested to the same standard. The results do not indicate the function of the unit under non standard or field conditions.

Product Description
The material consists of metal filings identified as KDF-55D. The granules are clean, shiny and brass colored. The formulation is proprietary.
Note: The following paragraph numbers refer to the corresponding paragraph numbers in NSF 53. Paragraphs not listed do not apply to this unit.

3. Materials

3.0 General: Materials which contact water shall be inert and not impart odor, color or taste to the effluent water. The materials and construction shall be appropriate for the use environment. Units intended for outdoor use shall not deteriorate because of sunlight.

Complies. See construction materials section.

3.1 Temperature resistance. Units, for cold water use only small not deteriorate when exposed to 100 degree F water. Units for hot water use shall withstand 180 degree F water for continuous use or 165 degree F if for intermittent hot water use.

Complies. The traits are not marked for restricted temperature use and were tested for mechanical properties at 180 degrees F.

3.2 Corrosion resistance: copper alloys, type 300 stainless, steels, Monel metal, and plastic materials (see 3.6) do not require a corrosion resistant coating.

Complies. See materials of construction section.

3.6 Plastics: Thermoplastic and thermoset plastics wetted by the water shall comply with the require ments of the U.S. PF&DA and /or NSF Standard 14.

Complies. See materials of construction section.

3.7 Joining materials shall meet the requirements of section 3.

Complies. See materials of construction section.

3.9 Gaskets, O Rings, Shaft Seals and Packing Materials shall meet the requirements of section 3.

Complies. See materials of construction section.

4. Design and Construction

4.0 General: The units shall be designed and constructed so that environmental influences such as vibration, shock, climactic conditions and the manufacturer's recommended cleaning procedures will not adversely affect them. , There shall be no burrs, sharp edges or other hazards which could cause injuries to users or mamienance personnel,

Complies.

4.0.1 Performance indication: The unit shall be provided with an effective alarm which will warn the user when the unit is no longer performing its design function. Acceptable warnings include:

4.0.1.3 Providing an obvious indication of the function's discontinuance, such as sharp reduction in flow or volumetric indication; or

4.4 Dispensing outlet: Faucets, spouts or outlets integral with the unit (except drinking outlets, see 4.5) shall be designed such that when the unit is installed as directed by the manufacturer the outlet will be directed downward and shall be a minimum of 1 inch above the flood level of the receptacle or sink.

Complies. The unit is provided with an industry standard diverter valve which is used to replace the aerator on the end of the tap spout. In normal installations, the diverter valve, although it extends approximately 3/8 inch further than the replaced aerator, should not decrease the distance to the high flood level below 1 ". In the case of spouts which do not have an aerator, and are not threaded, the diverter valve cannot be used. Responsibility for compliance with the applicable plumbing code is the responsibility of the installer.

Primary Drinking Water Regulations, or a similar standard established by any U.S. Federal regulatory agency for materials not included In the U.S. EPA regulations. No single sample shall exceed the MCL by more than the established analytical error for the method used.

5.2 Chemical Units: Chemical reduction shall be substantiated by tests showing compliance with the minimum requirements specified as follows:

5.2.1 Chemical reduction: Shall reduce the specified contaminants as shown in Table 1.

Complies. See Table 1

Note: Since it was known that the sample material has little, if any, effect on organic materials, the test was continued using the challenge water until any one of the metallic inorganic elemental components reached the maximum allowable effluent concentration.

Table 1

Concentration:
Chemical Concentration, Parts per million (mg/l) Input Water Unit Output Maximum Allowed
TTHM (as CHC13) 0.51 .051 <0.1
Lead (as PbC12) 0.16 .009 <0.025
Fluoride 8.20 6.88 <1.4
Nitrate (as N) 30.2 19.9 <10.0
Barium 10.0 .000** <1.0
Arsenic 0.33 .001 <0.05
Cadmium 0.030 .003 <0.01
Chromium VI 0 .152 .052 <0.05
Chromium 111 0.163 .065 <0.05
Selenium 0.105 .000** <0.01
Mercury 0.0063 .000** <0.002
Endrin 0.0008 .0008 <0.0002
Lindane 0.013 .013 <0.004
Methoxychlor 0.30 .030 <0.1
Toxaphene 0.016 .016 <0.005
2,4 D 0.30 .30 <0.1
Silvex (2,4,5 TP) 0.03.2 .032 <0.01
Copper 0.000* 5.88 *
Zinc 0.000* 8.99 *
Sulfur (As H2S) 0.50 .000**
Chlorine (As HCIO) 2.0 1.34


*No copper or zinc were added to the challenge water; no present acceptable levels are set for these elements, however it is anticipated that standards will be established.
**Below limit of detection

Resume general water test 510 14
ca day 4 ca 25 8 hr shutdown 500 13
ca 50 8 hr shutdown 510 13
ca 75 8 hr shutdown 510 18
ca 90 start 60 hr shutdown 510 16
restart test 500 2
ca 100 8 hr shutdown 520 15
ca 120 Start high TDS water test
80 vol + 8 hr shutdown
510 17
resume high TDS to 100 vols
Start low TDS water test
80 vol + 8 hr shutdown
510 20

5.1.2 Chemical: The arithmetic average of the active agent of each test unit shall not exceed the MCL of the U.S. EPA Primary Drinking Water Regulations, or a similar standard established by any U.S. federal regulatory agency for materials not included in the U.S. EPA regulations. No single sample shall exceed the MCL by more than the established analytical error for the method used.

5.2 Chemical Units: Chemical reduction shall be substantiated by tests showing compliance with the minimum requirements specified as follows:

5.2.1 Chemical Reduction: Shall reduce the specified contaminant/s as shown in Table 1.

Complies. See Table 1.

Note: The results given in this table are the readings at 100 percent of manufacturer's listed capacity.

Table 1

Concentration:
Chemical Concentration, Parts per million (mg/l) Input Water Unit Output Maximum Allowed
TTHM (as CHC13) 0.55 .002 <0.1
Lead (as PbC12) 0.16 .000** <0.025
Fluoride 8.00 1.32 <1.4
Nitrate (as N) 30.0 8.8 <10.0
Barium 10.0 .035 <1.0
Arsenic 0.32 .000** <0.05
Cadmium 0.030 .004 <0.01
Chromium VI 0.151 .011 <0.05
Chromium 111 0.153 .009 <0.05
Selenium 0.013 .002 <0.01
Mercury 0.0066 .000** <0.002
Endrin 0.0007 .0001 <0.0002
Lindane 0.012 .0001 <0.004
Methoxychlor 0.31 .003 <0.1
Toxaphene 0.015 .001 <0.005
2,4 D 0.30 .21 <0.1
Silvex (2,4,5 TP) 0.032 .001 <0.01
Copper 0.000* 3.32 *
Zinc 0.000* 7.99 *
Aluminum (AlCl3) 2.0 .93 ***
Sulfur (As H2S) 1.0 .000** ***
Chlorine (As Cl3) 3.0 .000** ***
Iron (FeCl3)*** 1.0 .000** ***

*No copper or zinc were added to the challenge water; no present acceptable levels are set for these elements, however it is anticipated that standards will be established.
"Below limit of detection.
***Added because of advertising claims.

5.4 Working pressure and hydrostatic performance.

5.4.1.1 Complete assemblies shall withstand a hydrostatic pressure equal to 2.4 times the working pressure or 300 psig, whichever is greater, for 15 minutes without leakage of water from the unit.

Complies. When pressurized to 300 psig at 180 degrees F, there was no failure or leakage of the end of 15 minutes at pressure.

5.4.1.5 Valves and controls subject to line pressure shall be water tight at 150 psig pressure after a minimum of 100,000 pressure cycles of 0 150 psig at 70 =/ 5 degrees F.
Does not apply. Although the unit is provided with a diverter valve which diverts the water flow from the faucet outlet to the filter, there is no closure valve on the outlet of the filter. No pressurization is intended of the system.

5.4.3 Disposable pressure vessels and all replaceable components subject to line pressure shall be constructed for a working pressure of at least 125 psig and shall meet the following requirements:

5.2 Chemical Units: Chemical reduction shall be substantiated by tests showing compliance with the minimum requirements specified as follows:

5.4.3.1 Withstand a hydrostatic pressure of 2.4 times the working pressure or 300 psig, whichever is greater, for 15 minutes without leakage.

Complies. See paragraph 5.4.1.1 above.

5.4.4 Units designed for open discharge, including connecting tubing where required shall be con structed for a working pressure of at least 125 psig and the following requirement:

5.4.4.1 Complete assemblies, with the outlet plugged, shall withstand a hydrostatic pressure of 1.2 times the working pressure of 150 psig, whichever is greater, for 15 minutes without leakage.

Complies.

General Performance

Burst test, non metallic pressure vessels

Minimum design burst pressure is equal to 4 times the working pressure or 500 psig, whichever is the greater.

Complies. Burst pressure: 933 psig

5.5 Filter media: All finer media which may be subject to plugging shall be supported to withstand for 15 minutes a pressure differential of 75 psig or a pressure differential equal to the maximum pressure drop the product design will permit when installed under a line pressure of 125 psig without breakdown which would permit the passage of untreated water or media into the effluent.

Complies.

Filter Media Test
Input turbidity: 11 TU
Effluent turbidity, initial* 0.7 TU
Effluent turbidity, 50 % 0.8 TU
Requirement: Effluent turgidity to be 1.0 TU or less; Effluent shall contain no media.
*The unit was purged prior to test to remove carbon dust.

5.6 Rated pressure drop: In line entire system units shall not have more than a 10 psig initial pres sure drop at the rated service flow. This does not apple if the unit has an integral flow control or orifice. These units shall meet the requirements of 5.7.

Rated Pressure Drop
Pressure drop: 2.1 psi

5.7 Rated service flow: 1 gpm

5.7.1 The rated service flow shall be as specified by the manufacturer.

Complies. Measured flow 1.32 gpm

6 instruction and information

6.0 Operation, maintenance and installation instructions must comply with the requirements of this

section.

Complies. Installation instructions are provided; there are no user maintenance requirements.

6.1 The units must be labeled in compliance with the requirements of this section.

6.3 Purchase and sales information must comply with the requirements of this section.

Complies.



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